Dielectrophoretic motion of oblate spheroidal particles. Measurements of motion of red blood cells using the Stokes method

作者: J M Cruz , F J García-Diego

DOI: 10.1088/0022-3727/31/14/020

关键词:

摘要: A theoretical expression for the dielectrophoretic velocity of oblate spheroidal cells is presented in this paper. The cell has been measured using Stokes method and a V-shaped electrode chamber calculated as function cell's position. Human red blood (RBC) were used to verify model. Velocity measurements performed frequency range between 150 kHz 3 MHz suspending medium conductivity 2 mS . parameter that includes properties RBC, dimensions (the length long semiaxis axial ratio) electrical real component cell-medium Clausius-Mossotti factor, defined. values are corrected polarization effects take place at electrode-medium interface. good agreement experimental observations predictions we found validates theory. value can be very sensitive tool detect small differences size changes their shape properties.

参考文章(24)
X.B. Wang, Y. Huang, X. Wang, F.F. Becker, P.R. Gascoyne, Dielectrophoretic manipulation of cells with spiral electrodes Biophysical Journal. ,vol. 72, pp. 1887- 1899 ,(1997) , 10.1016/S0006-3495(97)78834-9
P R C Gascoyne, Ying Huang, R Pethig, J Vykoukal, F F Becker, Dielectrophoretic separation of mammalian cells studied by computerized image analysis Measurement Science and Technology. ,vol. 3, pp. 439- 445 ,(1992) , 10.1088/0957-0233/3/5/001
K. Asami, T. Hanai, Dielectric monitoring of biological cell sedimentation Colloid and Polymer Science. ,vol. 270, pp. 78- 84 ,(1992) , 10.1007/BF00656933
J. Gimsa, T. Müller, T. Schnelle, G. Fuhr, Dielectric spectroscopy of single human erythrocytes at physiological ionic strength: dispersion of the cytoplasm Biophysical Journal. ,vol. 71, pp. 495- 506 ,(1996) , 10.1016/S0006-3495(96)79251-2
Y Huang, X -B Wang, J A Tame, R Pethig, Electrokinetic behaviour of colloidal particles in travelling electric fields: studies using yeast cells Journal of Physics D: Applied Physics. ,vol. 26, pp. 1528- 1535 ,(1993) , 10.1088/0022-3727/26/9/030
D.C. Chang, Cell poration and cell fusion using an oscillating electric field. Biophysical Journal. ,vol. 56, pp. 641- 652 ,(1989) , 10.1016/S0006-3495(89)82711-0
R Pethig, Ying Huang, Xiao-bo Wang, J P H Burt, Positive and negative dielectrophoretic collection of colloidal particles using interdigitated castellated microelectrodes Journal of Physics D. ,vol. 25, pp. 881- 888 ,(1992) , 10.1088/0022-3727/25/5/022
N.J. Rivette, J.C. Baygents, A note on the electrostatic force and torque acting on an isolated body in an electric field Chemical Engineering Science. ,vol. 51, pp. 5205- 5211 ,(1996) , 10.1016/S0009-2509(96)00337-5
Tomakazu Matsue, Norio Matsumoto, Shinji Koike, Isamu Uchida, Microring-ring electrode for manipulation of a single cell. Biochimica et Biophysica Acta. ,vol. 1157, pp. 332- 335 ,(1993) , 10.1016/0304-4165(93)90118-R