Proteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae)

摘要: Rapidly growing, green motile flagellates of Haematococcus pluvialis can transform into enlarged red resting cysts (aplanospores) under oxidative stress conditions. However, it is not known what initial molecular defense mechanisms occur in response to stress, and may ultimately lead cellular transformation. In this study, global-expression profiling proteins was analyzed by two-dimensional gel electrophoresis, image analysis, peptide mass fingerprinting. Oxidative induced cultures addition acetate Fe2+, exposure excess light intensity. Overall, 70 were identified with altered expression patterns following induction. Some key involved photosynthesis nitrogen assimilation down-regulated, whereas some mitochondrial respiratory transiently up-regulated after the onset stress. Most proteins, particularly those from families superoxide dismutase, catalase, peroxidase, up-regulated, but reverted down-regulation during 6 days On other hand, accumulation antioxidant astaxanthin occurred well initiation reached its maximum level six or more It appears that early involves multiple enzymatic processes play a critical role upon also transition vegetative cells cysts. As cyst development continues, intensive, enzyme-mediated responses largely replaced mature astaxanthin. This study provides first direct evidence for massive, concerted up-regulation antioxidative mechanisms, both spatially temporarily, protect H. against