Distribution of Tetracycline Resistance Gene, tet(M), in Gram-Positive and Gram-Negative Bacteria Isolated from Sediment and Seawater at a Coastal Aquaculture Site in Japan

摘要: We found increased numbers of oxytetracycline (OTC)-resistant bacteria in sediment and seawater around a marine aquaculture site after OTC therapy. Samples were collected at an along the coast Seto Inland Sea, Japan 2004. In April, percentage resistant to 60 μg mL-1 surface was 6.8%-20.0%. The percentages during therapy summer reaching 53.3%-60.7% September. Ninety-two days drug cessation, decreased below 22.9%. Tet(M)-positive detected samples. Tet(M) evident both Gram-positive Gram-negative from various genera, newly identified Paenibacillus, Sporosarcina, Shewanella, Pseudoalteromonas. dominant tet(M)-positive isolates strains Vibrio suggesting that this genus is important reservoir for tet(M) environment. Two different alleles found, tet(M)-A tet(M)-B, each five genera. data suggests used acted as selective pressure promoting bacteria.