The behaviour of the autonomous maize transposable element En/Spm in Arabidopsis thaliana allows efficient mutagenesis.

摘要: The behavior of the autonomous maize transposable element En/Spm was studied in Arabidopsis. Transgenic Arabidopsis plants carrying En-1 elements were propagated for 12 generations using a single seed descent procedure. distribution and activity monitored Southern DNA hybridisations 1, 6 12. In first generation highest number insertions per line 7, which increased to 20 average only slightly population, due gradual accumulation segregants that lost element. During development mutagenised population remained active even high-copy lines. situ hybridisation demonstrated multiple distributed over all chromosomes. From initial populations many unstable gene mutations recovered, indicating can be used as efficient tool tagging