The Ubap1 Subunit of Escrt-I Interacts with Ubiquitin Via a Souba Domain.
作者： Monica Agromayor , Nicolas Soler , Anna Caballe , Tonya Kueck , Stefan M. Freund
DOI: 10.1016/J.STR.2011.12.013
关键词:
摘要: The endosomal sorting complexes required for transport (ESCRTs) facilitate of ubiquitinated cargo, MVB biogenesis, late stages cytokinesis, and retroviral budding. Here we show that ubiquitin associated protein 1 (UBAP1), a subunit human ESCRT-I, coassembles in stable 1:1:1:1 complex with Vps23/TSG101, VPS28, VPS37. X-ray crystal structure the C-terminal region UBAP1 reveals domain describe as a solenoid overlapping UBAs (SOUBA). NMR analysis shows each three rigidly arranged making up SOUBA interact ubiquitin. We demonstrate UBAP1-containing ESCRT-I is essential degradation antiviral cell-surface proteins, such as tetherin (BST-2/CD317), by viral countermeasures, namely, HIV-1 accessory Vpu Kaposi sarcoma-associated herpesvirus (KSHV) ligase K5.
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