Cytokines as Diagnostic Biomarkers in Canine Pyometra and Sepsis

摘要: Sepsis is a syndrome with high morbidity, mortality and astronomical health care costs it challenging to diagnose both in humans animals due the lack of suitable diagnostic biomarkers. Although several types proteins have been suggested as biomarkers sepsis, none them were shown be reliable for routine use clinical practice. Dogs uterine bacterial infection called pyometra often develop sepsis natural model sepsis. To investigate whether there pattern that can useful on early stages addition existing criteria, we measured local gene expression serum levels cytokines dogs compared these known inflammatory markers blood clotting parameters. Serum concentrations keratinocyte-derived chemokine (KC)-like protein global clot strength significantly increased healthy without pyometra. Moreover, chemokines interleukin (IL)-8 C-X-C motif ligand 5 (CXCL5) mRNA higher uteri from cultured stromal endometrial cells derived cocultured LPS or pathogenic Escherichia coli unstimulated cells. IL-8, high-mobility group box 1 (HMGB1), prostaglandin F2α, IL-2, IL-15, IL-18, interferon (IFN)-γ monocyte-macrophage colony stimulating factor (MG-CSF) not different between presence pyometra, some correlated parameters such total white cell count (correlated HMGB1) KC-like IL-8). Measurements IL-10, CXCL10, tumor necrosis (TNF)-α, IL-6 IL-4 will require more sensitive method Our findings suggest KC-like, CXCL5 IL-8 may Further investigation help improve routines diagnosis possibly also humans.