Steroid Hormone Uptake by Anterior Pituitary Cell Suspensions11

摘要: Anterior pituitary glands from 7–day ovariectomized rats were serrated and digested with 0.05% pronase in calcium—magnesium free Hanks' medium (CMFH). Cells dispersed CMFH contained characteristic secretory granules had RNA/DNA protein DNA ratios similar to those of intact tissue. When a constant number cells was incubated increasing concentrations 3H-estradiol, uptake retention 3H-estradiol resulted specific nonspecific binding processes. The component high affinity (KA = 2.3 X 109M-1) low capacity for estradiol (0.0029 pmole/Mg DNA). lower not saturable using up 1.55 10-8M. Nonspecific greatly reduced when bound BSA before incubation cells. Scatchard plot data indicated there are ∼19000 sites per cell (10.8 pg Pituitary concentrated rapidly at 37 C, an...