BENZOIC ACID DERIVATIVE IN THE SECRETA OF THE GENITAL EPITHELIUM OF THE COW.

摘要: The secretion of citric acid by the bovine female genital epithelium has been investigated Gaspar & Cseh (1963). In 60% cases no was present, but in secreta 40 % cows, quantities corresponding to spots 1 \m=.\5to \g=m\gcould be demonstrated. 30 one unidentified spot, usually accompanied and showing Rf values from 0\m=.\78to 0\m=.\80,also appeared on chromatogram. We have trying identify more closely compound forming this spot. It diffusible remained unhydrolysed n-sulphuric for 2 hr character acid. Although unknown generally acid, it could not identified either with lactic pyruvic components citrate cycle, or maleinic citraconic Oestrous eighty-four living dairy cows sixty slaughtered animals were used as test material. material collected directly os cervix aspiration a syringe provided catheter. Uterine mucus, oviduct fluid, follicular fluid organ. method paper chromatography described Schreier Hack (1956) used. absorption spectrum stained compound, eluted ethanol, measured Beckman spectrophotometer. is supposed related even identical hippuric which found exceedingly large herbivora, final product detoxication benzoic its derivates. This conclusion based following considerations. (a) comparison made between chromatographic data Gaffney, Schreier, DiFerrante Altman (1954) our own results (see Table 1). (b) maximum substance tested Gaffney et al. at 460 m\g=m\,while that deriving under similar conditions 475 m\g=m\(see Fig. Text-figs. 2). (c) In-vivo load tests also prove derivative. Sixty 90 mg/kg sodium benzoate administered intravenously five cows. tentatively