Purification and properties of the ubiquitin-conjugating enzymes Cdc34 and Ubc13.Mms2.
作者： Christopher Ptak , Xaralabos Varelas , Trevor Moraes , Sean McKenna , Michael J. Ellison
DOI: 10.1016/S0076-6879(05)98005-1
关键词:
摘要: A prerequisite for structure/function studies on the ubiquitin‐conjugating enzymes (Ubc) Cdc34 and Ubc13·Mms2 has been ability to express purify recombinant derivatives of each. This chapter describes methods used in expression purification these proteins from Escherichia coli, including variations protocols generate 35S, 15N, 13C/15N, seleno‐L‐methionine derivatives. Assays measure Ub thiolester conjugation activities Ubcs are also described.
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