The Bmx Tyrosine Kinase Induces Activation of the Stat Signaling Pathway, Which Is Specifically Inhibited by Protein Kinase Cδ

摘要: Members of the hematopoietically expressed Tec tyrosine kinase family have an important role in hematopoietic signal transduction, as exemplified by crucial Btk for B-cell differentiation and activation. Although a variety cell surface receptors been found to activate kinases, specific signaling pathways substrate molecules used kinases are still largely unknown. In this study kinase, Bmx, was induce activation Stat pathway. Bmx induced phosphorylation DNA binding activity all factors tested, including Stat1, Stat3, Stat5, both mammalian insect cells. also transcriptional Stat1- Stat5-dependent reporter genes. Other cytoplasmic Syk, Fyn, c-Src, showed no or only weak ability proteins. Expression cells endogenous proteins without Jak kinases. We further analyzed Bmx-mediated which be regulated protein C δ (PKCδ) isoform, but not β 1, e, ζ isoforms, leading inhibition Stat1 phosphorylation. conclusion, these studies show that can function activator pathway identify PKCδ regulation signaling.