Gastric Mucosal Injury in the Rat

摘要: Recent studies have implicated oxygen free radicals in ischemia-reperf usion injury to the gastric mucosa. The aims of present study were (a) test hypothesis that enzyme xanthine oxidase is source ischemic stomach and (b) determine importance ironcatalyzed Haber—Weiss reaction in,generating cytotoxic radicals. Gastric mucosal clearance 5 'Cr-labeled red blood cells was measured during a 30-min control period, period (hemorrhage 25 mmHg arterial pressure), 60–80-min reperf (reinf shed blood). In untreated (control) rats, dramatic rise (100-fold) leakage 51 Cr-labeled into lumen observed only period. A f ter damage further assessed using gross lesion area histology. Rats placed on sodium tungstate diet (to inactivate oxidase), or treated with either de eroxamine (an iron chelating agent) superoxide dismutase (a scavenger). All three interventions substantially reduced cell relative rats. However, tissue histologically similar both animals. results this support mediate hemorrhagic shock-induced extravasation cells. data also indicate oxy-radicals largely responsible for hydroxyl radical generation model.