Entomological Surveillance and Detection of Dengue Viruses in Vector Mosquitoes as an Early Warning Tool for the Control of Dengue in Pakistan

摘要: Dengue viruses spread through the bites of female Aedes mosquitoes to human mostly in urban areas tropical/sub-tropical countries. is occurring as epidemic annually Pakistan since 2006. Recently 2011 a severe occurred Punjab province along with >15000 positive cases and > 400 deaths, especially highly populated city Lahore. With neither vaccine nor proper treatment for dengue, prevention disease depends upon surveillance early diagnosis/detection dengue virus antigens from mosquito vectors which can serve an warning system forecasting impending outbreaks. In current study 28 entomological surveys were carried out various localities Lahore March-September, collection mosquitoes. Two species aegypti albopictus found common during this period. However, present throughout these months while appeared July-August, 2011. addition types natural artificial breeding containers also observed immature stages mosquitos all localities, visited above mentioned The most productive automobiles used tyres larval production 94% positivity. Collected screened using specific monoclonal antibodies (MAB) antigen capture Enzyme Linked Immunosorbant Assays (ELISAs). Of 114 pools females (n=570) screened, 31 indicating 27.19% infection rate (MIR). 04 (n=40) screened; only 1 pool was 25% This first report DENV detection adult Ae.