Comparative bioinformatic and proteomic approaches to evaluate the outer membrane proteome of the fish pathogen Yersinia ruckeri.

摘要: Yersinia ruckeri is the aetiological agent of enteric redmouth (ERM) disease and responsible for significant economic losses in farmed salmonids. Enteric associated primarily with rainbow trout (Oncorhynchus mykiss, Walbaum) but its incidence Atlantic salmon (Salmo salar) increasing. Outer membrane proteins (OMPs) Gram-negative bacteria are located at host-pathogen interface play important roles virulence. The outer Y. poorly characterised little known about composition individual OMPs Here, we employed a bioinformatic pipeline to first predict OMP ruckeri. Comparative proteomic approaches were subsequently used identify those expressed vitro eight representative isolates recovered from trout. One hundred forty-one predicted four genomes 77 these identified three or more considered as “core” proteins. Gel-free gel-based together 65 single reference isolate subsequent gel-free analysis 64 isolates. Together, our analyses 84 unique