Hypoxia-dependent changes of enzyme activities in different fibre types of rat soleus and extensor digitorum longus muscles. A cytophotometrical study

作者: Karla Punkt , Antje Unger , Klaus Welt , Heidegard Hilbig , Lutz Schaffranietz

DOI: 10.1016/S0065-1281(96)80017-1

关键词:

摘要: Summary Using cytophotometry activity changes of succinate dehydrogenase, glycerol-3-phosphate dehydrogenase and myofibrillar adenosine triphosphatase were measured in 3 fibre types soleus extensor digitorum longus muscles under normal experimental conditions. Fibres typed by means cytophotometrical data into slow-oxidative, fast-oxidative glycolytic fast-glycolytic ones. After hypoxia 20 min duration a significant increase enzyme activities was observed especially slow-oxidative fibres both muscles, e.g. increased 21% these muscle 23–26% muscle. Moreover, an 10% 28% 10–12% ATPase all measured. Treatment with Ginkgo biloba extract for months before exposure to resulted decreased These results could be interpreted as protective effect extract.

参考文章(37)
K. Drieu, Preparation and definition of Ginkgo biloba extract Presse Medicale. ,vol. 15, pp. 1455- 1457 ,(1988) , 10.1007/978-3-642-73686-5_3
Singal Pk, Kirshenbaum La, Changes in antioxidant enzymes in isolated cardiac myocytes subjected to hypoxia-reoxygenation. Laboratory Investigation. ,vol. 67, pp. 796- 803 ,(1992)
KEITH VEITCH, ANNICK HOMBROECKX, LOUIS HUE, Ischaemia/anoxia-induced increases in cardiac mitochondrial respiration: changes in Complex I. Biochemical Society Transactions. ,vol. 19, ,(1991) , 10.1042/BST019261S
Hiromi TAKAHASHI, Kunio KIKUCHI, Hideaki NAKAYAMA, Effect of Chronic Hypoxia on Oxidative Enzyme Activity in Rat Skeletal Muscle. The Annals of physiological anthropology. ,vol. 12, pp. 363- 369 ,(1993) , 10.2114/AHS1983.12.363
P. Hochachka, Muscle enzymatic composition and metabolic regulation in high altitude adapted natives. International Journal of Sports Medicine. ,vol. 13, ,(1992) , 10.1055/S-2007-1024606
C. Vetter, H. Reichmann, D. Pette, Microphotometric determination of enzyme activities in type-grouped fibres of reinnervated rat muscle. Histochemistry and Cell Biology. ,vol. 80, pp. 347- 351 ,(1984) , 10.1007/BF00495415
C. E. Blanco, G. C. Sieck, V. R. Edgerton, Quantitative histochemical determination of succinic dehydrogenase activity in skeletal muscle fibres. Histochemical Journal. ,vol. 20, pp. 230- 243 ,(1988) , 10.1007/BF01747468
Hiroaki Takekura, Toshitada Yoshioka, Determination of metabolic profiles on single muscle fibres of different types Journal of Muscle Research and Cell Motility. ,vol. 8, pp. 342- 348 ,(1987) , 10.1007/BF01568890