High-throughput bioanalytical LC/MS/MS determination of benzodiazepines in human urine: 1000 samples per 12 hours.

摘要: The analytical capabilities of liquid chromatography tandem mass spectrometry for sensitive and highly selective determination target compounds in complex biological samples makes it well suited high-throughput analysis. We report the fast separation six benzodiazepines isolated from human urine via selected reaction monitoring chromatography/mass using short dwell times to accommodate fast-eluting chromatographic peaks. analytes were extracted along with their deuterium-labeled internal standards by a simple liquid−liquid extraction 96-well plates. Using four autosamplers coupled one column spectrometer operated turbo ion spray mode positive detection, 1152 (12 plates) analyzed less than 12 h. Through an electronic switching box designed constructed in-house, synchronized so that injections made as soon th...