Requirement of the Pr55gag precursor for incorporation of the Vpr product into human immunodeficiency virus type 1 viral particles.

摘要: The human immunodeficiency virus type 1 (HIV-1) particles consists of two molecules genomic RNA as well originating from gag, pol, and env products, all synthesized precursor proteins. 96-amino-acid Vpr protein, the only virion-associated HIV-1 regulatory is not part polyprotein precursors, its incorporation into must occur by way an interaction with a component normally found in virions. To investigate mechanism virion, Vpr- proviral DNA constructs harboring mutations or deletions specific gene products were cotransfected expressor plasmids COS cells. Virus released transfected cells was tested for presence immunoprecipitation Vpr-specific antibodies. results these experiments show that trans-incorporated virions but at lower efficiency than when expressed construct. minimal viral genetic information necessary deleted provirus encoding pr55gag precursor. Incorporation requires expression processing gag independent pol expression. Direct Pr55gag protein demonstrated coprecipitation product-specific Overall, indicate incorporated nascent virion through Gag demonstrate novel which can be particles.