Modular Poly(ethylene glycol) Matrices for the Controlled 3D-Localized Osteogenic Differentiation of Mesenchymal Stem Cells

摘要: The in vitro formation of physiologically relevant engineered tissues is still limited by the availability adequate growth-factor-presenting cell-instructive biomaterials, allowing simultaneous and three-dimensionally localized differentiation multiple tissue progenitor cells. Together with ever improving technologies such as microfluidics, printing, or lithography, these biomaterials could provide basis for generating provisional cellular constructs, which can differentiate to form mimetics. Although state-of-the-art are endowed sophisticated modules time- space-controlled positioning release bioactive molecules, reports on 3D arrangements differentiation-inducing growth factors scarce. This paper describes stable immobilization biotinylated molecules a modular, Factor XIII-cross-linked poly(ethylene glycol) hydrogel platform using genetically streptavidin linker. Linker incorporation demonstrated Western blot, functionality confirmed capturing alkaline phosphatase (ALP). After optimizing bone morphogenetic protein 2 (BMP-2) biotinylation, streptavidin-modified hydrogels able bind present BMP-2-biotin. Finally, this scheme BMP-2, specific osteogenic mesenchymal stem cells inducing ALP expression confined areas. In future, together other affinity-based strategies will be useful local various engineering cell-responsive constructs.