Angiotensin-I converting enzyme inhibitory and antioxidant activities of protein hydrolysates from Phaseolus lunatus and Phaseolus vulgaris seeds

摘要: Abstract Phaseolus lunatus and vulgaris protein concentrates were hydrolyzed with the enzymes Alcalase® Flavourzyme® at different reaction times, angiotensin-I converting enzyme (ACE-I) inhibitory activity, antioxidant properties amino acid composition measured in hydrolysates. With Alcalase®, highest degree of hydrolysis (DH) P. was 37.94% 45 min, 49.48% 30 min. Flavourzyme®, DH's 22.03% 26.05%, respectively, both 90 min. ACE-I activity hydrolysates IC50 = 0.056 mg mL−1 for 90 min, IC50 = 0.061 mg mL−1 60 min. In this IC50 = 0.0069 mg mL−1 90 min IC50 = 0.127 mg mL−1 45 min. SDS-PAGE, exhibited low molecular weight bands. Antioxidant 11.55 mmol L−1 TEAC mg−1 10.09 mmol Amino high hydrophobic residues content.