Étude thermodynamique de l'interaction de la proflavine avec l'acide desoxypribonucleique: I. Étude par equilibre de dialyse

摘要: Abstract Thermodynamic study on the interaction between proflavin and DNA I. Equilibrium dialysis studies The thermodynamics of first complex (2,8-diaminoacridine) with have been studied by equilibrium in range 0° to 70° at three ionic strengths 0.01, 0.1 1.0. number binding sites decreases when temperature or strength increases. enthalpy reaction is not constant but T . This effect can be interpreted basis a thermally reversible change state DNA, transition which about 40° markedly different from so-called melting DNA. total free energy ΔG ° may considered as sum terms = s + e H , where specific characterises permanent dipoles base pairs induced polarisable dye. electrostatic solvation energy, corresponds “dehydration” both partners rehydration bound system. In case highest (1.0), are quite negligible therefore equal whose value 6.4 kcal. As expected theoretical considerations, entropy was found 11 kcal/mole·degree. All results favour intercalation model.