Influence of NG-nitro-L-arginine methyl ester on clinical and biochemical effects of methylene blue in pentylenetetrazole-evoked convulsions Uticaj NG-nitro-L-arginin metil estra na klini˛ ke i biohemijske efekte metilen plavog kod konvulzija izazvanih pentilentetrazolom

摘要: Background/Aim. Despite years of research in a number experimental models the question whether nitric oxide (NO) and methylene blue (MB) have pro- or anticonvulsant effects remains to be fully resolved. Methods. In adult Wistar rats influence nonselective inhibitor synthase NG-nitro-L-arginine methyl ester (L- NAME, 10 �¨ g) on clinical biochemical MB (10 given before intraperitoneally administered chemical convulsant pentylenetetrazole (PTZ, 80 mg/kg) was examined. L-NAME were applied intracere- broventricularly. PTZ application followed by 4- minute observation time, after which sacrificed elements oxido-reductive balance measured crude mitochondrial fraction forebrain cortex, hippo- campus striatum. Results. Convulsive responses (fore- limb dystonia - FLD, generalised clonic- clonic-tonic convulsions GCC GCTC respectively) observed all received PTZ, together with significantly de- creased lipid peroxidation cortex stria- tum increased superoxide dismutase activity hip- pocampus, comparison controls (saline treated). It registered pretreatment. However, these insignificant. hippocam- pus animals there decreased (p < 0.01, p 0.05 vs saline-treated PTZ-treated rats, re- spectively) reverted PTZ-induced increase superox- ide activity. But individually pretreatment incidence CTCs GCCs (FLD: = 0.0513), prolonged convulsive latent time for GCTCs GCCs, examined brain regions level su- peroxide anion. Administration minutes be- fore MB-evoked effects. Conclusion. Methylene has strong that eliminated These changed parameters brains treated MB- group suggest involvement NO MB's animal model PTZ-evoked convulsions.