Posttranscriptional Control of Gene Expression in Yeast

摘要: Studies of the budding yeast Saccharomyces cerevisiae have greatly advanced our understanding posttranscriptional steps eukaryotic gene expression. Given wide range experimental tools applicable to S. and recent determination its complete genomic sequence, many key challenges control field can be tackled particularly effectively by using this organism. This article reviews current knowledge cellular components mechanisms related translation mRNA decay, with emphasis on molecular basis for rate regulation. Recent progress in characterizing factors their protein-protein RNA-protein interactions has been rapid. Against background a growing body structural information, review discusses thermodynamic kinetic principles that govern process. As prokaryotic systems, translational initiation is point control. Modulation activities imposes global regulation cell, while features particular 5′ untranslated regions, such as upstream open reading frames effector binding sites, allow gene-specific data revealed new details involved providing insight into functional overlaps networking are apparently feature evolving systems. An overall picture governing decay only very recently begun develop. The latest work information about pathways, degradation machinery, way which these might relate apparatus. Overall, major still addressed include task relating compartmentalization polysome structure role channelling highly complex expression