Rabies virus dissemination in neural tissues of autopsy cases due to rabies imported into Japan from the Philippines: Immunohistochemistry

摘要: Two Japanese men, 65 and 69 years old, developed rabies in Japan around 2-3 months after dog-bite exposure the Philippines. Laboratory diagnosis of was made following detection virus genome on reverse transcription-polymerase chain reaction from saliva, immunohistochemistry a nuchal skin punch biopsy one case. The patients died 9 19 days clinical onset. At autopsy, no macroscopic changes CNS were observed. Histopathology indicated that eosinophilic cytoplasmic inclusion bodies, Negri seen neuronal cells CNS. Inflammatory cell reactions scarce, apoptosis detected. Immunohistochemistry demonstrated nucleoprotein (N) phosphoprotein (P) disseminated to all neural tissues body with similar pattern both cases. Interestingly, there differences localization between N P antigen brain, but located at peripheral nerve sheaths localized axons. These data indicate dissemination causes disease development death. for is powerful tool understand pathogenesis rabies.