Cytochrome P-450 and NADPH cytochrome c reductase in rat brain: formation of catechols and reactive catechol metabolites.
作者： Henry A. Sasame , Matthew M. Ames , Sidney D. Nelson
DOI: 10.1016/0006-291X(77)90510-1
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摘要: Microsomes isolated from whole rat brain were found to contain cytochreme P-450 (0.025 0.051 nmoles/mg) and NADPH cytochrome c reductase activity (26.0 55.0 nmoles/mg/min). The oxidation of estradiol a reactive metabolite that became covalently bound microsomal protein was inhibited 63% by an atmosphere CO:O2 (9:1), indicating the involvement oxygenase. In contrast, this had no effect on binding either catechol estrogen, 2-hydroxyestradiol, or several catecholamines microsomes. An antibody prepared against decrease significantly both formation 2-hydroxyestradiol microsomes covalent estrogen protein.
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