The cochaperone shutdown defines a group of biogenesis factors essential for all piRNA populations in Drosophila.

摘要: In animal gonads, PIWI proteins and their bound 23–30 nt piRNAs guard genome integrity by the sequence specific silencing of transposons. Two branches piRNA biogenesis, namely primary processing ping-pong amplification, have been proposed. Despite an overall conceptual understanding identity and/or function involved players are largely unknown. Here, we demonstrate essential role for female sterility gene shutdown in biology. Shutdown, evolutionarily conserved cochaperone collaborates with Hsp90 during potentially at loading step RNAs into proteins. We that Shutdown is both secondary populations Drosophila. An extension our study to previously described pathway members revealed three distinct groups biogenesis factors. Together data on how wired processing, propose a unified model biogenesis.