Purification and Biological Characterization of Shiga Toxin from Shigella dysenteriae 1
作者： J. Edward Brown , Darrell E. Griffin , Sara W. Rothman , B. P. Doctor
DOI: 10.1128/IAI.36.3.996-1005.1982
关键词:
摘要: Shiga toxin has been purified in milligram quantities to near homogeneity from cell lysates of Shigella dysenteriae 1 strain 3818-0. Purification involved an initial ultracentrifugation, ammonium sulfate fractionation, chromatography on DEAE-cellulose and carboxymethyl cellulose, gel filtration, preparative isoelectric focusing sucrose gradients. The was resolved by discontinuous polyacrylamide electrophoresis into a major cytotoxic protein band closely migrating, protease-nicked minor band. Antiserum generated immunization with glutaraldehyde-inactivated shown be monospecific against S. lysates. This highly HeLa cells, enterotoxic rabbit ileal loops, lethal mice. Monospecific antiserum the neutralized completely these activities both preparations crude shigella
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