De novo transcriptome assembly of Angelica dahurica and characterization of coumarin biosynthesis pathway genes.

摘要: Abstract Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. Sav (A. dahurica) is a famous Chinese herb known for the production of coumarins, important secondary metabolites with wide-ranging pharmacological activities. In particular, methoxylated coumarins like those produced by A. are their anti-inflammatory, anti-cancer, and anti-oxidant effects. However, molecular mechanism coumarin biosynthesis in has not been studied. Such investigation could help scientists harness potential coumarins. Here we present, three transcriptomes corresponding to leaf, root, stem tissues dahurica. A total 114,310 unigenes an average length 1118 bp were de novo assembled, 81,404 (71.21%) annotated. Then, 181 encoding seven key enzymes involved identified, which COMT (Caffeic acid 3-O-methyltransferase) was spatially used phylogenetic analysis, some these enzyme genes verified qRT-PCR. Differentially expressed root-specific-expressed comparing genes’ profile activity between roots other tissues. Furthermore, multiple or transcription factors related identified analyzed. This study first report comprehensive gene information at transcriptional level, distinguish candidate its coumarin, thus laying foundation this pathway’s further exploration