T-cell subset analysis of peripheral T-cell lymphomas by paraffin section immunohistology and correlation of CD4/CD8 results with flow cytometry.

摘要: Peripheral T-cell lymphomas (PTCLs) are often diagnosed after demonstration of T-lineage-related antigen expression on neoplastic lymphocytes by paraffin immunoperoxidase (PIP). However, complete subset analysis for helper, suppressor/cytotoxic, alphabeta, and gammadelta phenotypes has not been examined PIP. Therefore, PIP was performed CD4, CD8, intracellular (TIA)-1, betaF1 in 31 PTCLs previously studied CD4 CD8 flow cytometry. The results from both methods were compared. All betaF1- receptor (TCR)gammadelta showed 71% correlation with the 21 that had distinct CD4+ CD8- or CD4- CD8+ cytometry, 64% 90% sensitivity expression, respectively. Tumor cells four six no clear predominance coexpression these antigens cytometry shown to be Twelve (39%) demonstrated a cytotoxic (TIA-1+) phenotype PIP, including eight CD8+, one three cases. Of 30 immunoreactive PTCLs, 26 (87%) alphabeta (betaF1+) Both large cell cases among TCRgammadelta+ gammadelta+ case confirmed We conclude subsets can assigned most showing moderate strong cytometric results. also define tumor which produces inconclusive Cytotoxic identified easily TIA-1, distinguish "suppressor" PTCLs. Most derived T-cells, however some may