Sugar receptors of the stromal cell layer in human long-term bone marrow cultures: Their presence, modulatory responses to changes in the microenvironment and potential role in cellular adhesion

摘要: Intimate cellular contacts and coordinated supply of regulatory factors are required to maintain the still inexplicable dynamic equilibrium hemopoiesis. To infer potential participation protein-carbohydrate interaction in this complex process, human long-term bone marrow cultures were initiated from eleven donors, adherent cell layer was characterized enzyme- immunohistochemically. Utilizing an array carrierimmobilized carbohydrate ligands sulfated polysaccharides as probes, specific binding various constituents chains glycoconjugates stromal cells unmistakably disclosed. Biochemical analysis, employing glycocytologically effective affinity chromatography, corroborated result. The extent markedly lower two samples, derived leukemia patients. Pronounced adaptive responses for characteristic followed changes culture microenvironment that known influence qualitative quantitative aspects hemopoiesis vitro, namely omission hydrocortisone horse serum or addition cytokines. Similarly, such modulation occurred on level accessible surface receptors, monitored by neoglycoenzymes. These sites can be involved mediation interactions, revealed a model system interference N-acetyl-D-galactosamine adhesion. Overall, results support idea glycobiological recognition may contribute functional integrity well provide basis further analysis.