Stabilization of cationic liposome‐plasmid DNA complexes by polyamines and poly(ethylene glycol)‐phospholipid conjugates for efficient in vivo gene delivery

摘要: Stable complexes of cationic liposomes with plasmid DNA were prepared by (1) including a small amount poly(ethylene glycol)-phospholipid conjugate or (2) condensing the polyamines prior to formation liposome-plasmid complexes. These preparations stable for months at 4°C and gave reproducible high transfection activity in vivo gene delivery after intravenous injection mice. Under these conditions, expression marker (luciferase) was primarily lungs (reaching values up 3 ng per mg tissue protein), but also other tissues lesser extent. Non-stabilized formulations lost all their 4 days. In cholesterol, not dioleoylphosphatidylethanolamine, helper lipid effective sustaining vivo. new developments formulation technology should enhance potential liposome-mediated therapy.