KLEBSIELLA PNEUMONIAE RESISTANT TO THIRD GENERATION CEPHALOSPORINS IN A HOSPITAL IN VENEZUELA: SPREAD OF ESBLPRODUCING ST70 AND ST1261 CLONES

摘要: Objectives. To characterize K. pneumoniae isolates presenting decreased susceptibility to third generation cephalosporins, and isolated in two high risk units of the University Andes Hospital (HULA). Methods. We studied all strains non-susceptible cephalosporins from patients environment Neonatal High Risk Unit (NHRU) Adult Intensive Care (AICU) HULA Merida, Venezuela, February March 2007, June November 2009. Antibiotic was tested by diskdiffusion method E-test method. Detection blaβLEE blaAmpC genes carried out PCR sequencing. The genetic relationship between established pulsed field gel Electrophoresis (PFGE) Multilocus Sequence Typing (MLST). Results. In total 39 were identified, 30 them NHRU 9 AICU; 20 (51,28%) produced CTX-M-15, 8 (20,51 %) CTX-M-2, (20,51%) SHV-12, 2 (5,12%) SHV-1 overproduction 1 (2.56%) EBC. By PFGE, ten clusters found. MLST, more prevalent PFGE belonged ST70, (here reported for first time Venezuela), ST126. Conclusions. Its seem be time, dissemination ST70 clone producing CTX-M-15 different LAUH is being described Venezuela; also this worldwide description ST1261 CTX-M-2 SHV-2.