Stimulation of Human Dendritic Cells by Wild-Type and M Protein Mutant Vesicular Stomatitis Viruses Engineered To Express Bacterial Flagellin

摘要: Vesicular stomatitis viruses (VSVs) containing wild-type (wt) or mutant matrix (M) proteins are being developed as candidate vaccine vectors due to their ability induce innate and adaptive immunity. Viruses with wt M protein, such recombinant (rwt) virus, stimulate maturation of dendritic cells (DC) through Toll-like receptor 7 (TLR7) its adaptor molecule MyD88. However, protein viruses, rM51R-M both TLR7-positive TLR7-negative DC subsets. The goal this study was determine whether the rwt human can be enhanced by engineering these express bacterial flagellin. Flagellin expressed from virus genome partially protected VSV-induced shutoff host synthesis promoted production interleukin 6 (IL-6) IL-1β. In addition, infected expressing flagellin were more effective at stimulating gamma interferon (IFN-γ) CD8+ allogeneic T than virus. Although effectively stimulated DC, cytokines. Furthermore, mice immunized had anti-VSV antibody responses in vivo. Therefore, may promising for delivery foreign antigen potential function.