Complementary precursor ion and neutral loss scan mode tandem mass spectrometry for the analysis of glycerophosphatidylethanolamine lipids from whole rat retina

摘要: A "shotgun" tandem mass spectrometry (MS) approach involving the use of multiple lipid-class-specific precursor ion and neutral loss scan mode experiments has been employed to identify characterize glycerophosphatidylethanolamine (GPEtn) lipids that were present within a crude lipid extract normal rat retina, obtained with minimal sample handling prior analysis. Characterization these was performed by complementary analysis their protonated deprotonated ions, as well various ionic adducts (e.g., Na(+), Cl(-)), using triple-quadrupole spectrometer. Notably, application novel scans m/z 164 43, respectively, for specific identification sodiated GPEtn ions following addition 500 microM NaCl extracts demonstrated. The MS/MS in parallel provided simplified spectra enhanced detection 1-alkenyl, 2-acyl (plasmenyl) relative positive 141 commonly used Furthermore, "low energy" experiment monitor exclusive 36m/z (HCl) from [M+Cl](-) demonstrated provide more than 25-fold enhancement negative Subsequent "high-energy" pseudo MS(3) product on identified this then rapidly fatty acyl chain substituents lipids.