Packed bed enzyme microreactor: Application in sucrose hydrolysis as proof-of-concept

摘要: Abstract A continuous flow enzyme microreactor was designed, assembled and run that allowed for operation with immobilized enzymes in particulate form. As a proof-of-concept, invertase covalently bound to silane-coated silica carriers used the hydrolysis of sucrose into invert sugar syrup. Once glutaraldehyde solution load were optimized, kinetic behavior biocatalyst established, particles loaded sandwich type microreactor, resulting packed bed The fed substrate within 1.0–8.0% (w/v), at rates ranging from 17.5 259.0 μL/min. Apparent kinetics evaluated using Lilly–Hornby model. Accordingly Km(app) values decreased increasing rates, whereas, higher tends stabilize close observed free Full conversion up 8.0% (w/v) sucrose. Moreover, formulation displayed high operational stability, as it retained roughly 100% its initial activity during 30 days.