Vardenafil prevents fibrosis and loss of corporal smooth muscle that occurs after bilateral cavernosal nerve resection in the rat

摘要: Abstract Objectives Impotence, specifically corporal veno-occlusive dysfunction (CVOD), occurs after radical prostatectomy. It results from the effects of cavernosal nerve damage, which causes smooth muscle (SM) loss and an increase in collagen within corpora. Recent reports have suggested that long-term treatment with phosphodiesterase-5 inhibitors prostatectomy may prevent such changes. We aimed to determine whether bilateral resection (BCNX) rat leads CVOD inhibition ameliorates these histologic functional impairments. Methods Rats (n = 7 11/group) underwent either sham operation, BCNX, or BCNX plus 30 mg/L vardenafil drinking water. Before rats were killed 45 days later, was assessed by dynamic infusion cavernosometry. The corpora histochemistry/immunohistochemistry quantitative image analysis for SM/collagen ratio, III/I alpha-SM actin, inducible nitric oxide synthase (iNOS), proliferating cell nuclear antigen, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling as a marker apoptosis. Results Compared group, demonstrated measured drop rate, 60% reduction twofold iNOS expression, threefold intracorporeal increased both antigen expression (SM replication), normalization cavernosometry rate ratio. Conclusions Long-term preserving SM content inhibiting fibrosis possibly its effect on iNOS.