Derivation of Human Embryonic Stem Cells from Blastocysts
作者： Biljana Dumevska , Julia Schaft , Ulrich Schmidt
DOI: 10.1016/B978-0-12-385473-5.00036-9
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摘要: In this chapter we focus on protocols for the handling of embryos from stage when they are thawed to point deemed an embryonic outgrowth and cryopreserved. This will emphasize methods directly related embryos, outgrowths, putative hESC lines. includes embryo culture after thawing, plating, initial outgrowths. The actual in vitro fertilization (IVF) including freezing thawing is covered Chapter 35, as ethical issues that surround use generation stem cells.
sci-hub.se 参考文章(7)
Jeanne Frances Loring, Robin L Wesselschmidt, Philip H Schwartz, Human Stem Cell Manual: A Laboratory Guide ,(2007)
Irina Klimanskaya, Young Chung, Sandy Becker, Shi-Jiang Lu, Robert Lanza, Human embryonic stem cell lines derived from single blastomeres Nature. ,vol. 444, pp. 481- 485 ,(2006) , 10.1038/NATURE05142
Cara K. Bradley, Heather A. Scott, Omar Chami, Teija T. Peura, Biljana Dumevska, Uli Schmidt, Tomas Stojanov, Derivation of Huntington's disease-affected human embryonic stem cell lines. Stem Cells and Development. ,vol. 20, pp. 495- 502 ,(2011) , 10.1089/SCD.2010.0120
J. A. Thomson, Embryonic Stem Cell Lines Derived from Human Blastocysts Science. ,vol. 282, pp. 1145- 1147 ,(1998) , 10.1126/SCIENCE.282.5391.1145
B.E. Reubinoff, M.F. Pera, G. Vajta, A.O. Trounson, Effective cryopreservation of human embryonic stem cells by the open pulled straw vitrification method. Human Reproduction. ,vol. 16, pp. 2187- 2194 ,(2001) , 10.1093/HUMREP/16.10.2187
J. W. Catt, M. Henman, Toxic effects of oxygen on human embryo development. Human Reproduction. ,vol. 15, pp. 199- 206 ,(2000) , 10.1093/HUMREP/15.SUPPL_2.199
Teija T. Peura, Julia Schaft, Tomas Stojanov, Derivation of human embryonic stem cell lines from vitrified human embryos. Methods of Molecular Biology. ,vol. 584, pp. 21- 54 ,(2009) , 10.1007/978-1-60761-369-5_2