Proteomics-Based Analysis of Phalaenopsis amabilis in Response toward Cymbidium Mosaic Virus and/or Odontoglossum Ringspot Virus Infection

摘要: Stress response at the protein level to viral infection in orchid plants has not been extensively investigated date. To understand proteomic basis of Phalaenopsis amabilis’s responses Cymbidium Mosaic virus (CymMV), and/or Odontoglossum ring spot (ORSV), total proteins were extracted from amabilis leaves infected with CymMV, ORSV, or both respectively. Differentially expressed identified by two-dimensional electrophoresis, and 27 these that had significant changes further examined by mass spectrometry. Comparing CymMV-infected leaves mock-inoculated ones, 2 significantly up-regulated, 9 down-regulated and 1 previously undetected was identified. 10 were significantly 3 down-regulated previously undetected ORSV-infected leaves. 6 up-regulated were found co-infected These are involved disease resistance, stress response, transcriptional regulation, energy metabolism, protein modification unknown known pathways. Proteins ATP sulfurylase, included glutamate decarboxylase isozyme 2, RNA polymerase alpha subunit chloroplastic peptide deformylase 1A similar alteration trend after all infection treatments. Significantly Thioredoxin H-type down-regulated Cytosolic phosphoglycerate kinase I which were proteins have been shown be specifically regulated CymMV. Significantly like Rubisco large subunit, Triosephosphate isomerase, NADP-specific isocitrate dehydrogenase Cinnamoyl CoA reductase CCR2 ORSV. Protein regulation coinfected leaves followed a pattern any single results. The