Nitrergic signalling via interstitial cells of Cajal regulates motor activity in murine colon.

摘要: In the enteric nervous systems, NO is released from nitrergic neurons as a major inhibitory neurotransmitter. acts via NO-sensitive guanylyl cyclase (NO-GC), which found in different gastrointestinal (GI) cell types including smooth muscle cells (SMCs) and interstitial of Cajal (ICC). The precise mechanism signalling through these two to regulate colonic spontaneous contractions not fully understood yet. present study we investigated impact endogenous exogenous on contractile motor activity using mice lacking nitric oxide-sensitive (NO-GC) globally specifically SMCs ICC. Longitudinal proximal colon wild-type (WT) knockout (KO) mouse strains exhibited ex vivo. WT muscle-specific (SMC-GCKO) showed an arrhythmic with varying amplitudes frequencies. contrast, global ICC-specific (ICC-GCKO) animals regular rhythm constant duration amplitude rhythmic contractions. Nerve blockade (tetrodotoxin) or specific (L-NAME, ODQ) did significantly affect GCKO ICC-GCKO whereas patterns SMC-GCKO were transformed into uniform patterns. response electric field-stimulated neuronal release was similar GCKO. conclusion, our results indicate that basal myenteric ICC influence generation effects elevated are mediated by murine colon.