Therapeutic proteins : methods and protocols

摘要: Biopharmaceutical Proteins: Opportunities and Challenges John R. Birch Yemi Onakunle Expression of Antibody Fragments by Periplasmic Secretion in Escherichia coli Andrew G. Popplewell, Mukesh Sehdev, Mariangela Spitali, A. Neil C. Weir Secretory Production Therapeutic Proteins Sang Yup Lee, Jong Hyun Choi, Jun Lee Recombinant LTB Protein Marine Vibrio VSP60 Wang Yili Si Lusheng Heterologous Gene Yeast J. Byrne, Kenneth O'Callaghan, Mick F. Tuite Pharmaceutical from Methylotrophic Yeasts Eric de Bruin, Erwin H. Duitman, Arjo L. Boer, Marten Veenhuis, Ineke Bos, Erik Hack Human Papillomavirus Type 16 L1 Baculovirus Systems: A Case Study Zheng Jin, Lusheng, Large-Scale Transient Mammalian Cells Sabine Geisse, Martin Jordan, Florian M. Wurm Site-Specific Integration for High-Level Bhaskar Thyagarajan Michele P. Calos Suspension Culture Lily Chu, Ilse Blumentals, Gargi Maheshwari Transgene Control Engineering Beat Kramer Fussenegger Fusion to Albumin as a Means Slow the Clearance Small Using Pichia pastoris System: William Sheffield, Teresa McCurdy, Varsha Bhakta High Throughput Recovery Inclusion Bodies coli: An Overview Amulya K. Panda Isolation, Solubilization, Refolding, Chromatographic cells: Surinder Singh, N. S. Eshwari, Lalit Garg, Preparation Factor VIIa Plasma: Teruhisa Nakashima Kazuhiko Tomokiyo Purification Clinical-Grade Monoclonal Antibodies Methods Alberto Horenstein, Ilaria Durelli, Fabio Malavasi Virus Elimination Validation Nicola Boschetti Anna Johnston Removal Nanofiltration Marina Korneyeva Scott Rosenthal Determining Residual Host Cell Antigen Levels Purified Slot Blot Scanning Laser Densitometry Aaron Miles, Daming Zhu, Allan Saul Principles Formulation: Sellers Yuh-Fun Maa Solid-State Methodologies, Stability, Excipient Effects Stabilization Chemical Physical Ken-ichi Izutsu Extraction Characterization Vaccine Antigens Water-In-Oil Adjuvant Formulations Miles Probing Reversible Self-Association Sedimentation Velocity Analytical Ultracentrifuge Bernardo Perez-Ramirez Steckert Biological Pegylated Interferons: Lei Xie, Constance Cullen, Sheri Bradshaw, Marc DeLorenzo, Michael Grace Interferon a2B Via Carboxyalkylation: David Wylie, Marcio Voloch, Seoju Yan-Hui Liu, Collette Cutler, Brittany Larkin, Susan Cannon-Carlson Quantifying Their Degradation Products SDS-PAGE Denaturing Gel Electrophoretic Methodology Analysis F