DEVELOPMENT AND VALIDATION OF LC METHOD FOR THE ESTIMATION OF DISOPYRAMIDE IN PHARMACEUTICAL DOSAGE FORM

摘要: ABSTARCT A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed validated for the estimation of Disopyramide in tablet dosage form. An Inertsil ODS C-18, 5µm column having 250 x 4.6mm internal diameter isocratic mode with mobile containing Methanol: Acetonitrile: THF ratio 55 : 45 5 ,(v/v/v) used. The flow rate 1.0ml/min effluents were monitored at 265nm. retention time 2.967 min. linearity, accuracy, precision, specificity, limit detection, quantification robustness. Limit detection found to be 0.03ppm 0.099ppm respectively recovery from formulation 98.87%. proposed successfully applied quantitative determination formulation.