Empleo del Vitrofural en la esterilización química del endospermo artificial de los embriones somáticos encapsulados de Saccharum spp. híbrido var Cuba 87- 51

作者: Mayra Acosta , Daniel Agramonte , Elisa Quiala , Elio Jiménez , Yelenys Alvarado

DOI:

关键词:

摘要: The effect of the Vitrofural in control pollutants capsule study was carried out, as well its toxic on somatic embryos sugar cane. Different dose this compound used (20, 25, 30, 35 and 40 mg l-1), these they were applied to cultivation medium with nudes embryos, which previously autoclaved without autoclaved, later same doses added synthetic endosperm. can be employed chemical sterilization for germination vitro protection endosperm, that allowed eliminate encapsulation medium, reduce concentration agents gelificantes 33% carry out all operations under non sterile conditions. Key words: Encapsulation, sugarcane, seed

参考文章(9)
Yuji Sakamoto, Noboru Onishi, Takayasu Hirosawa, Delivery systems for tissue culture by encapsulation Automation and environmental control in plant tissue culture. pp. 215- 243 ,(1995) , 10.1007/978-94-015-8461-6_10
Beatriz M. Januzzi Mendes, Augusto Tulmann, Maria Cristina Falco, CELL SUSPENSION CULTURE OF SUGARCANE: GROWTH, MANAGEMENT AND PLANT REGENERATION1 ,(1996)
Fernando Payán A, Gilberto Tascón, Horacio Carmen, Técnicas para la micropropagacion de la caña de azúcar (Saccharum officinarum L.) mediante el cultivo de tejidos y yemas Acta Agronómica. ,vol. 27, pp. 43- 79 ,(1977)
E. Guiderdoni, Y. Demarly, Histology of somatic embryogenesis in cultured leaf segments of sugarcane plantlets Plant Cell Tissue and Organ Culture. ,vol. 14, pp. 71- 88 ,(1988) , 10.1007/BF00041181
Keith Redenbaugh, Brian D. Paasch, James W. Nichol, Mary E. Kossler, Peter R. Viss, Keith A. Walker, Somatic Seeds: Encapsulation of Asexual Plant Embryos Nature Biotechnology. ,vol. 4, pp. 797- 801 ,(1986) , 10.1038/NBT0986-797
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
Don J Heinz, Grace W. P. Mee, Plant Differentiation from Callus Tissue of Saccharum Species1 Crop Science. ,vol. 9, pp. 346- 348 ,(1969) , 10.2135/CROPSCI1969.0011183X000900030030X
J.M. Blondeau, N. Castanedo, O. Gonzalez, R Mendina, E. Silveira, In vitro evaluation of G1: a novel antimicrobial compound International Journal of Antimicrobial Agents. ,vol. 11, pp. 163- 166 ,(1999) , 10.1016/S0924-8579(98)00086-7
K. Redenbaugh, P. Viss, J.A. Fujii, D. Slade, Encapsulation of somatic embryos in synthetic seed coats HortScience (USA). ,(1987)