Molecular characterization of Acidovorax citrulli strain NIHHS15-280 causing bacterial fruit blotch disease in Korea and screening of resistance sources in melon

作者: Md Rafiqul Islam , Mohammad Rashed Hossain , Hoy-Taek Kim , Ujjal Kumar Nath , Md Abuyusuf

DOI: 10.1007/S13580-019-00190-0

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摘要: Bacterial fruit blotch (BFB), caused by Acidovorax citrulli, is an economically devastating disease in melon. Molecular characterization of causal strains and identification sources resistance are necessary to control this important disease. Many BFB were identified partial sequencing documented GenBank. Recently, there was outbreak strain NIHHS15-280 melon fields (Goksung, Jeonnam, Korea) that severely hampered production Korea. For reason, we characterized partially the 16S rRNA gene using PCR-based markers. The sequence much more similar A. citrulli associated with watermelon hosts than other cucurbit hosts. A assay (NIHHS15-280) as belonging group I, which predominantly infects non-watermelon cucurbits (virulent melon). Thirty-five genotypes screened against a detached-leaf inoculation assay. lack visible symptoms 11 days after lines PI353814 PI140471 indicated these be highly resistant lines, whereas moderate observed PI420145 SCNU1154 cultivars ME6 ME5. Also, most corroborated (group I) will serve valuable resource for further genetic studies improvement

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