Triterpene synthases from the Okinawan mangrove tribe, Rhizophoraceae.

摘要: Oleanane-type triterpene is one of the most widespread triterpenes found in plants, together with lupane type, and these two types often occur same plant. Bruguiera gymnorrhiza (L.) Lamk. Rhizophora stylosa Griff. (Rhizophoraceae) are known to produce both triterpenes. Four oxidosqualene cyclase cDNAs were cloned from leaves B. R. by a homology-based PCR method. The ORFs full-length clones termed BgbAS (2280 bp, coding for 759 amino acids), BgLUS (2286 761 RsM1 acids) RsM2 (2316 bp 771 ligated into yeast expression plasmid pYES2 under control GAL1 promoter. Expression GIL77 resulted production beta-amyrin lupeol, suggesting that genes encode lupeol synthase (LUS), respectively. Furthermore, produced germanicol, beta-amyrin, ratio 63 : 33 4, whereas taraxerol, proportions 70 17 13. This result indicates multifunctional synthases. Phylogenetic analysis sequence comparisons revealed demonstrated high similarities (78-93%) synthases, located branch as synthase. formed new was closely related cluster, first evolved Based on product profiles, we discuss molecular evolution synthases involvement formation terpenoids mangrove leaves.