Stepping into the third dimension.
作者： D. Feng , D. Marshburn , D. Jen , R. J. Weinberg , R. M. Taylor
DOI: 10.1523/JNEUROSCI.2846-07.2007
关键词:
摘要: Recent advances in optical-sectioning microscopy, along with novel fluorescent proteins and probes, give us the tools to image molecules their interactions space time. Investigators using these routinely collect multichannel three-dimensional (3D) images time series, but analyzing such complex datasets requires sophisticated visualization techniques. We here provide an overview of principles practices 3D microscopic data. also describe ImageSurfer, a new software package for volume data analysis. ImageSurfer is freely available (www.imagesurfer.org) provides powerful interactive explore analyze datasets. Although designed microscopy mind, it effective other types data, including acquired by functional magnetic resonance imaging EM tomography.
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