Effects of a Mixture of Volatile Organic Compounds on Total DNA and Gene Expression of Heat Shock Proteins in Drosophila melanogaster
作者： Oguzhan Doganlar , Zeynep Banu Doganlar
DOI: 10.1007/S00244-014-0089-4
关键词:
摘要: The genotoxic effects of a mixture 13 volatile organic compounds (VOCs) on total DNA profiles and the expression heat shock proteins (HSPs) HSP26, HSP60, HSP70, HSP83 in fruit fly tissues were examined. Drosophila melanogaster Oregon R+, reared under controlled conditions artificial diets, was treated with VOCs commonly found water at concentrations 10, 20, 50, 75 ppb for 1 5 days. Band changes clearly detected random amplified polymorphic assay, especially 50- 75-ppb exposure levels, both treatment periods. In addition, there clear differences band untreated flies intensity loss/appearance bands. Although genomic template stability (GTS) exhibited irregular first day, significant decreases GTS observed after days VOC application. lowest value (27.77 ± 1.96 %) level treatment. Quantitative real-time polymerase chain reaction analysis showed increase relative HSP26 HSP60 treatment, respectively. HSP70 increased significantly all times. However, greatest (4.2-fold) occurred 20 least affected by to VOCs. We conclude that trace levels can exert HSP Drosophila.
springer.com
sci-hub.se 参考文章(70)
Pavel Rössner, Blanka Binková, Radim J Šrám, Heat shock proteins hsp32 and hsp70 as biomarkers of an early response?: In vitro induction of heat shock proteins after exposure of cell culture to carcinogenic compounds and their real mixtures Mutation Research-genetic Toxicology and Environmental Mutagenesis. ,vol. 542, pp. 105- 116 ,(2003) , 10.1016/J.MRGENTOX.2003.09.005
J. A. Ryan, L. E. Hightower, Stress proteins as molecular biomarkers for environmental toxicology EXS. ,vol. 77, pp. 411- 424 ,(1996) , 10.1007/978-3-0348-9088-5_28
HELGA ECKWERT, GERD ALBERTI, HEINZ-R KOHLER, The induction of stress proteins (hsp) in Oniscus asellus (Isopoda) as a molecular marker of multiple heavy metal exposure: I. Principles and toxicological assessment Ecotoxicology. ,vol. 6, pp. 249- 262 ,(1997) , 10.1023/A:1018682928839
R. I. Morimoto, B. S. Polla, U. Feige, I. Yahara, Stress-Inducible Cellular Responses ,(2011)
Marco Maroni, Bernd Seifert, Thomas Lindvall, Indoor air quality : a comprehensive reference book Elsevier Science. ,(1995)
John M. Warrick, H.Y. Edwin Chan, Gladys L. Gray-Board, Yaohui Chai, Henry L. Paulson, Nancy M. Bonini, Suppression of polyglutamine-mediated neurodegeneration in Drosophila by the molecular chaperone HSP70. Nature Genetics. ,vol. 23, pp. 425- 428 ,(1999) , 10.1038/70532
M. J. Wolf, H. Amrein, J. A. Izatt, M. A. Choma, M. C. Reedy, H. A. Rockman, Drosophila as a model for the identification of genes causing adult human heart disease Proceedings of the National Academy of Sciences of the United States of America. ,vol. 103, pp. 1394- 1399 ,(2006) , 10.1073/PNAS.0507359103
Annamaria Piano, Paola Valbonesi, Elena Fabbri, Expression of cytoprotective proteins, heat shock protein 70 and metallothioneins, in tissues of Ostrea edulis exposed to heat and heavy metals. Cell Stress & Chaperones. ,vol. 9, pp. 134- 142 ,(2004) , 10.1379/483.1
Zeynep Banu Doğanlar, Oğuzhan Doğanlar, Kıymet Tabakçıoğlu, Genotoxic Effects of Heavy Metal Mixture in Drosophila melanogaster: Expressions of Heat Shock Proteins, RAPD Profiles and Mitochondrial DNA Sequence Water, Air, & Soil Pollution. ,vol. 225, pp. 2104- ,(2014) , 10.1007/S11270-014-2104-9
D Norback, E Bjornsson, C Janson, J Widstrom, G Boman, Asthmatic symptoms and volatile organic compounds, formaldehyde, and carbon dioxide in dwellings. Occupational and Environmental Medicine. ,vol. 52, pp. 388- 395 ,(1995) , 10.1136/OEM.52.6.388