Diverse, yet-to-be-cultured members of the Rubrobacter subdivision of the Actinobacteria are widespread in Australian arid soils.

摘要: Phylogenetic analyses of ribosomal RNA gene sequences (rDNAs) retrieved from an Australian desert soil sample (Sturt National Park) revealed the presence a number clones which branched deeply high GC Gram-positive division line descent. The most abundant group these were related to Rubrobacter. An oligonucleotide probe was designed have broad specificity Rubrobacter and relatives. This used interrogate eight rDNA libraries representing four distinct land forms within arid zone. Relative abundance Rubrobacter-relatives in samples ranged 2.6 10.2%. Clones selected for sequence analysis on basis heteroduplex mobility assay maximise diversity represented sample. Rubrobacter-related reported literature show strong support three groups. Database-searching ‘Rubrobacteria’ relatively published but absent others. A PCR group-1 test their 21 environmental samples. Only marine arid-zone gave positive results. Taken together results indicate are widespread variable diversity.