Investigation of miR-21, miR-141, and miR-221 in blood circulation of patients with prostate cancer

摘要: In addition to their potential as tissue-based markers for cancer classification and prognostication, the study of microRNAs (miRNAs) in blood circulation is also interest. present study, we investigated amounts three cancer-related miRNAs, miR-21, -141, -221 plasma prostate (PCa) patients. A cohort 51 patients with PCa was enrolled into miRNAs were measured two subgroups, localized/local advanced or metastatic PCa. group 20 healthy individuals served control group. quantified from total RNA fraction using 200 μl small molecule RNU1A a normalizing miRNA circulation. We found similar levels subjects median values 0.039, 0.033 0.04, respectively; (p = n.s.). patients, higher, miR-21 being highest (median, 1.51). The miR-221 intermediate 0.71) while miR-141 displayed lowest 0.051). differences between highly significant < 0.001; area under curve (AUC), 88%) AUC, 83%) but not 0.2). diagnosed PCa, all significantly higher than disease where difference most pronounced 75.5%). conclusion, analysis reveals varying patterns these molecules clinical subgroups