A new approach to monitoring proteolysis phenomena using antibodies specifically directed against the enzyme cleavage site on its substrate.

摘要: Proteolysis is a generic biochemical process that central in all biological activities. A new strategy for monitoring this proposed here. This approach based on the production of rabbit polyclonal anti-peptide antibodies directly against cleavage site substrate enzyme responsible proteolysis. So long as molecule's intact, antibody will bind to protein. However, after peptide bond by protease, no longer be able recognize substrate. Thus, development an ELISA uses specific allows hydrolysis protein monitored. Hydrolysis beta-casein plasmin, main indigenous protease milk, during ripening Swiss-type cheese, has been chosen model study.