The emerging contribution of sequence context to the specificity of protein interactions mediated by PDZ domains

摘要: The canonical binding mode of PDZ domains to target motifs involves a small interface, unlikely fully account for PDZ-target interaction specificities. Here, we review recent work on sequence context, defined as the regions surrounding not only but also their motifs. We address theoretical problem defining core and practical issue designing constructs. Sequence context is found introduce structural diversity, impact stability solubility constructs, deeply influence affinity specificity, thereby increasing difficulty predicting PDZ-motif interactions. expect that will have similar importance other protein interactions mediated by globular short linear