Extraction, PCR amplification and sequencing of mitochondrial DNA from human hair shafts

摘要: Techniques have been developed for extracting, amplifying and directly sequencing mitochondrial DNA (mtDNA) from human hair shafts. The shaft is ground in a glass micro-tissue grinder, the extracted with organic solvent purified by filtration. filtrate subsequently provides mtDNA template PCR. two hypervariable segments of control region are amplified four separate reactions. After purification step to remove unincorporated PCR primers, products quantitated capillary electrophoresis subjected cycle sequencing. separated analyzed on an automated sequencer. sequences match blood samples taken same donor.