Utilization of DNA-protein cross-links as a biomarker of chromium exposure.

摘要: Human exposure to carcinogenic Cr(VI) compounds is found among workers in a large number of professional groups, and it can also occur through environmental pollution. A significant toxic waste sites contain Cr as major contaminant. In this paper we summarize our efforts apply measurements DNA-protein cross-links (DPC) test for biologically active doses Cr(VI). DPC were at elevated levels lymphocytes several human populations with low medium exposures. At high Cr(VI), exemplified by group Bulgarian chromeplaters, plateaued adducts' similar those environmentally exposed individuals. Lymphocytic correlated strongly erythrocytes that are indicative exposure. not confounded such variables smoking, age, body weight, gender, or ethnicity. new version the cross-link assay offers improved sensitivity requires small amount biologic material. Preliminary results indicate ability reach detectable could be related lack repair these lesions lymphoid cells. Cr(III)-mediated DNA peptide glutathione single amino acids mutagenic cells, relationship higher molecular weight peptide/amino acid correlating more potent response. We speculate bulky have promutagenic effect. The current methodology does allow specific determination Cr-induced DPC; however, demonstrated assay's sample capacity may used initial screening occurrence damage Cr(VI)-exposed populations.